A searchable image resource of Drosophila GAL4-driver expression patterns with single neuron resolution

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A searchable image resource of Drosophila GAL4-driver expression patterns with single neuron resolution
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Team releases 74,000 fruit fly brain images for neuroscienceresearch elife

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale.

The footprint of this image resource exceeds our known current practical limits on standard public data repositories. Thus, we have made all the primary data used in this study freely available under a CC BY 4.0 license at https://doi.org/10.25378/janelia.21266625.v1 and through the publicly accessible website https://gen1mcfo.janelia.org. The images are made searchable with the same permissions on the user-friendly NeuronBridge website https://neuronbridge.janelia.org.

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