The Diagnostics Development Hub (DxD Hub), a national platform hosted by the Agency for Science, Technology and Research (A*STAR), Singapore, in collaboration with the National Institute for Environmental Studies (NIES), Japan, and the A*STAR Bioinformatics Institute (A*STAR BII), has successfully developed Steadfast, an advanced diagnostic kit...
Agency for Science, Technology and Research , SingaporeNov 22 2024 The Diagnostics Development Hub , a national platform hosted by the Agency for Science, Technology and Research , Singapore, in collaboration with the National Institute for Environmental Studies , Japan, and the A*STAR Bioinformatics Institute , has successfully developed Steadfast, an advanced diagnostic kit for detecting the highly pathogenic H5N1 Avian Influenza Virus .
HPAI strains, which are highly lethal and spread throughout the body of poultry, require immediate culling of affected flocks to prevent severe outbreaks. In contrast, LPAI strains are limited to the respiratory and gastrointestinal tract and cause mild symptoms, allowing for less drastic measures, such as enhanced monitoring and biosecurity.
DxD Hub played a key role in the development of the multiplex assay prototype test kits, including the design and development of avian clinical testing protocols, data analysis, and assay optimisation. Validated by NIES, Japan's primary laboratory for avian influenza testing in wild birds, the kit accurately classified avian influenza viruses detected in wild birds in Japan between 2022 and 2024 as either high or low pathogenicity.
Research Assay Avian Influenza Bioinformatics Biosecurity Diagnostic Diagnostics Gastrointestinal Tract Infectious Diseases Influenza Mortality Mutation Pandemic Public Health Respiratory Technology Virus
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