This article compares Solid Phase Extraction (SPE) results from traditional loose-packed plates and new composite plates which blend porous plastic with chromatographic SPE resin thus addressing channeling and inconsistent resin mass.
Sponsored Content by Porvair Sciences LimitedReviewed by Andrea SalazarAug 28 2024 Solid-phase extraction is the gold standard for sample preparation in chromatographic analysis. It is used to capture, clean, and concentrate samples while removing interfering compounds, helping users attain reliable and sensitive chromatographic data.
The following experiment compares SPE results from a C18 10 mg loose packed plate with a C18 10 mg composite plate , both created using the same batch of C18 resin.Methodology A range of compounds consisting of acidic, basic, and neutral analytes were selected based on their intrinsic physical and chemical properties, such as pKa and LogP. Testing with a wide range of compounds enabled a more detailed comparison between each plate format.
Reproducibility The difference in reproducibility between the two plates was substantial; a significant improvement was evident when using the composite plate. Figure 3 shows the composite silica plate had markedly higher reproducibility for all 11 compounds tested. Poorer recovery and reproducibility in the loose packed plate can be attributed to inconsistencies in the packing of chromatographic media, which is inherent to this process .
Conclusion The 10 mg Microlute® CSi C18 composite silica plate for SPE offered excellent reproducibility and recovery for all the compounds assessed in this study. The uniformity of the composite technology enables an even distribution of active media and maximizes interactions throughout the structure, resulting in exceptionally reproducible data.
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