Berry-flavored vapes impair lung immune response

Immune Response News

Berry-flavored vapes impair lung immune response
ActinAlveolar MacrophageBacteria
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Alterations in AM function following the exposure to berry-flavored e-cigarettes that lead to poor immune responses to pathogens and reduced host survival.

By Dr. Liji Thomas, MDReviewed by Benedette Cuffari, M.Sc.Nov 7 2024 Exposure alters immune cell movement and reduces pathogen clearance, increasing the risk of severe lung infections.

The innate immune cell signature of the lungs includes cells that regulate the immune response to pathogens like Pseudomonas aeruginosa, which ultimately induce inflammatory cytokines to prevent destructive lung inflammation. Typically, these bacteria are detected, phagocytosed, and cleared by AMs.

In the current study, researchers examined how inhaled berry-flavored e-cigarette vapor affects AM behavior. AM movement was monitored with PKH26, a membrane dye that stains for phagocytes. AM squeezing was reduced to 9%, whereas probing behavior increased to 75%. Increased probe length was also observed, which suggests reduced interalveolar migration in exposed mice. Patrolling behavior dropped from 33% to 21% after exposure.

The adverse effects of BFEV exposure on AM function were reflected in increased bronchoalveolar lavage fluid turbidity and surfactant A post-exposure. However, the berry flavor influenced changes in actin localization and intensity. BFEV exposure also reduced CDC42 levels, a protein that facilitates actin polymerization in the perinuclear area for cell migration.

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Actin Alveolar Macrophage Bacteria Capillaries Cell Cigarette Cytokine Cytokines Cytoplasm Inflammation Lungs Macrophage Membrane Nicotine Protein Respiratory

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