Staff at the North Cave Wetlands site in East Yorkshire say they have found a number of birds dead.
The black-headed gull is on the Amber conservation list, meaning there is "moderate concern" over its decline in numbersAn outbreak of avian flu is suspected at a nature reserve after a number of birds were found dead.
The trust said it was monitoring the situation and there were no plans to shut its reserves to the public. "We are also investigating and responding to other potential cases on our reserves across the region, including Potteric Carr.
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