This article introduces the Absorbance 96 App, a revolutionary tool designed to streamline and enhance the ELISA (enzyme-linked immunosorbent assay) workflow, focusing on the crucial readout stage.
Sponsored Content by ByonoyApr 19 2024Reviewed by Maria Osipova The ELISA is a valuable and adaptable tool for researchers in global biochemistry, molecular biology, and diagnostics. It is a simple and efficient assay for identifying antigens in biological specimens. The choice of ELISA is dependent on the specific research requirements. They all share four fundamental steps: coating, blocking, detection, and readout.
Step 1: Intuitively define workflow parameters for ELISA assay The software lets users choose the required reference and sample wavelengths for signal detection. The ability to choose the “reference wavelength” for sequential signal measurement provides more flexibility and insights. The need to deal with lengthy calculations or complicated Excel sheets is eliminated. Users can also input curve parameters manually with one click from a previous assay’s standard curve.
Figure 2. You control your plate layout and define samples, controls, and blanks as you like in a user-friendly way. Image Credit: Byonoy The results are provided in a variety of formats. The plate view format provides a summary of the optical density values for every well on the EILSA plate. The graph view visually displays the data determined by a preselected evaluation technique.
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