Discovery gives insight into brain function, breakdowns nature
showing overlap of channels following post hoc channel alignment. Active zone fluorescence profiles were assessed using a 100 nm-wide rectangle drawn through the center of Bassoon-labeled puncta, extending from the presynaptic terminal to the postsynaptic cell.) Averaged fluorescence profiles of Bassoon puncta imaged in 3 channels, showing the full-width at half maximum of gaussian fits to each channel, and the relative offset of fits to fluorescent peaks. .
The increase in FWHM with fluorophore wavelength is not expected to affect the localization analyses presented in Fig.) Representative calyx of Held from a WT mouse, immunolabelled for SYT3 and PSD-95 . Data are mean ± s.e.m. Error bands are obscured by the mean. SIM images used for fluorescence profiles were acquired using tissue from one WT animal, but similar results were obtained from 2 or more animals of each genotype while optimizing immunohistochemistry.
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