Genetic variant carried by 60% of Europeans predisposes people to slimness CNIO_Cancer
For mouse tissues and primary cells, RNA was extracted using TRIzol together with RNeasy Mini Kit or Direct-zol RNA Miniprep . Reverse transcription was performed using SuperScript IV VILO Master Mix . Quantitative real-time PCR was run in triplicates using GoTaq qPCR Master Mix in a QuantStudio 6 Flex Real-Time PCR System thermocycler . The 2as reference gene.
A final multifactorial model to predict BMI was validated through bootstrap, using 2000 bootstrap samples, which allowed to correct the performance statistics by optimism in order to assess its predictive power and possible overfit. This final model was obtained by sequentially adding SNP and gene expression variables to a basal model with only sex and age as predictor variables. A new variable was accepted if it was statistically significant and in addition produced a new model with an increased optimism-corrected, in order to prevent overfit. In the final model, the relative importance for the different predictor variables was estimated from the χAll tests were bilateral, with a significance level of 0.05.
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