This study investigates the upconversion luminescence properties of NaYF4:Yb,Er UCNPs using an Edinburgh Instruments FS5 Spectrofluorometer. The characterization includes spectral, time-resolved, and quantum yield assessments. The results reveal important information about the UCNPs' emission characteristics, lifetime, and efficiency, highlighting their potential for various applications.
Sponsored by Edinburgh InstrumentsReviewed by Andrea SalazarFeb 7 2025 Photon upconversion occurs when two or more lower-energy photons are converted into higher-energy ones. Upconversion nanoparticles are a key type of upconversion material.
Proper understanding of upconversion luminescence characteristics that belong to UCNPs is critical for customizing their features to specified use cases and maximizing their performance. In this report, an Edinburgh Instruments FS5 Spectrofluorometer was utilized for a full upconversion luminescence characterisation of UCNPs consisting of spectral, time-resolved, and quantum-yield assessments.
Figure 2. Upconversion emission spectrum of the UCNPs acquired using a 2 W 980 nm laser diode in CW mode and the PMT-900. Image Credit: Edinburgh Instruments Upconversion Lifetime Upconversion decay of the 4F9/2 → 4I15/2 transition at 653 nm was then assessed, as shown in Figure 4. The 980 nm laser diode was put into pulsed mode with a repetition rate of 500 Hz and a pulse width of 10 µs. Meanwhile, the upconversion decay was acquired via MCS single photon counting. The decay was next put in Fluoracle® via a two-exponential model, bringing about an intensity average lifetime of 125 µs.
Multiple reasons may be behind this, including the forbidden 4f – 4f transition, and unavoidable challenges such as poor crystallinity and surface defects.4 Developing UCNPS with elevated quantum yields is an ongoing research pursuit.
UPCONVERSION NANOPARTICLES SPECTROFLUOROMETRY QUANTUM YIELD BIOMEDICINE
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