Genetic loss of miR-205 in mice enhances mammary gland development and branching, challenging previous lethality findings and highlighting its onco-suppressive functions.
By Dr. Sanchari Sinha Dutta, Ph.D.Jul 30 2024 A study published in the journal Non-Coding RNA describes the involvement of microRNA-205 in mammary gland development in female mice.
MicroRNA-205 has also been found to be associated with mammary gland development. It is highly expressed in the basal epithelium but not the luminal compartment. Its expression increases in both basal and luminal epithelium during pregnancy and lactation. The involvement of microRNA-205 in mammary gland stem cell regeneration has also been evidenced.
Generation of mouse model The scientists generated a conditional microRNA-205 knockout mouse model using the well-described Cre-loxP technology. Using in situ hybridization technique, they confirmed the loss of microRNA-205 expression in the mammary gland of knockout mice. They further confirmed the knockout using Northern Blot and qRT-PCR methods.
They tested their hypothesis by analyzing microRNA-205 knockout mammary glands at different stages of development and found no significant structural and developmental defects. They found that microRNA-205 knockout female mice were perfectly able to feed their pups. Similarly, they observed that microRNA-205 knockout ducts have pseudostratified and hyperplastic epithelia with frequent mitoses at five months of age.
These findings highlight onco-suppressive functions of microRNA-205 that control cell proliferation and survival by regulating the expression of several targets.
Cancer Genetic Mammary Gland Cell Epidermis Gene Gene Expression Genes Growth Factor Hybridization Knockout Microrna Mouse Model Pregnancy Receptor RNA Signaling Pathway
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