New study offers insight into the development of human triple negative breast cancers BUMedicine NatureComms
; lsl-EYFP; K8CreERT2 mice displayed in figures was performed on mice collected 7 days after the last Tamoxifen dose, except where noted. Histology for Lats1/2; lsl-EYFP; Sox9CreERT2 mice; and lsl-EYFP; Sox9CreERT2 mice displayed in figures was performed on mice collected 5–6 days after the last Tamoxifen dose and 7 days after the last Tamoxifen dose, respectively..
. For FACS experiments, live/dead staining was performed using Calcein blue at a concentration of 1 µM. Cell sorting was performed on a Moflo Astrios flow cytometer .Ad5mK8-nlsCre was diluted in DMEM with CaCl2 and introduced into the fourth mammary ducts at a titer of ~1.92 × 10pfu/duct using 100 µL Nanofil syringes fitted with 35 G Nanofil needles . Live animal and organ imaging was performed using an IVIS Spectrum imaging system .
Gene set enrichment analysis was used to identify biological terms, pathways, and processes that are coordinately up- or down-regulated within each pairwise comparison. The Entrez Gene identifiers of the human homologs of all genes in the Ensembl Gene annotation were ranked by the Wald statistic computed for each pairwise comparison.
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