An interview with Till Myrach, Product Manager at Knauer, on the principles and applications of osmolality measurement using the K-7400S osmometer.
Osmometry is a method to determine a sample's osmolality, which is the total number of osmotically active particles solved in a solution. These particles influence the colligative properties of the solution, including boiling and freezing point but also the vapor pressure. While the boiling point is elevated with an increasing number of particles in a solution, the other two are lowered.
The fascinating point about osmolality is that it depends on the number of solute particles but not on their chemical nature. Therefore, it can be considered as a value representing the total concentration of solutes. A field of application is the quality control of isotonic beverages. Knauer’s K-7400S semi-micro osmometer uses the principle of freezing point osmometry to determine the osmolality of sample liquids. In a first step, the osmometer rapidly cools the sample from room temperature to below 0 °C. Although the temperature of the sample falls even below its actual freezing point, the solution remains liquid. At a predefined temperature, freezing is then initiated by a small movement of a wire that dips into the sample. This turbulence results in a uniform crystallisation and a subsequent temperature equilibration at the freezing point of the sample. During the entire process, the sample's temperature is precisely monitored by a high-resolution thermistor
OSMOMETRY K-7400S KNAUER FREEZING POINT OSMOMETRY QUALITY CONTROL
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