Researchers discover key pathway for COVID organ damage in adults EmoryUniversity NatureComms
ProteomicsPatient samples were de-identified prior to receipt and subsequent experimentation and analysis performed blinded. All reagents were of HPLC grade or higher. Plasma samples were thawed on ice and gently mixed by agitating the side of the tube. The 10 µL of neat plasma was diluted 1:10 in 100 mM triethylammonium biocarbonate . Protein concentration was then determined by performing a Bradford protein assay.
statistical analysis was done using pairwise protein-based abundance with the ratio of COVID positive/negative control samples. A Benjamini-Hochberg correction for FDR was applied and adjustedFor all lipidomics and metabolomics experiments, patient samples were de-identified prior to receipt. The subsequent analysis of lipids was blinded. All patient samples were processed in a cold room at 4 °C.
software was used to process raw data files and identify features in the samples. Lipids were annotated with LipidSearch 4.2 software . Only MS2 level confirmed lipid species grade A, B, and, C were used and lipids with grade D or lower were removed.
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