Ash Regan, Alba's sole MSP, must hope 25 other members at Holyrood support her motion or it will not proceed to a vote.
Patrick Harvie faces a potential vote of no confidence following his comments over the landmark Cass review into gender identity services for young people.
Asked five times if he accepts the report, the minister refused to say, instead claiming it has been "politicised and weaponised" against trans people. Regan - who defected from the SNP to Alba last year - said: "The motion of no confidence speaks for itself. The Scottish Greens wish to side with ideology over clinical evidence.
The motion creates an awkward position for some SNP MSPs who are known for their gender critical stance and antipathy to the Greens.
Scottish Green Party Gender Issues Ash Regan
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