Research challenges 'sugar hypothesis' of diabetic cataract development brighamwomens
]. Pigmented Long-Evans rats were purchased . Animals were maintained in a temperature-controlled environment with a 12 h dark/light cycle and provided access to standard chow and water ad libitum.The surgical microscope Pentero 900 was used to visualize the pathological changes in the lenses of live NGRs.
The following antibodies were used: goat anti-E-Cadherin , rabbit anti-MAGP1 , rabbit anti-CD45 , rabbit anti-CD68 and secondary antibodies . An in situ cell death detection kit was used for the TUNEL assay.To investigate trafficking of immune cells to the lens, we developed an in situ anterior half-globe microscopy technique. Eyes were snap frozen, OCT-embedded, and gradually cut from back toward the front to reach the height of the ciliary bodies, using a cryostat .
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