Production of anti-SARS-CoV-2 antibodies from hyperimmunized chickens Antibodies Coronavirus Disease COVID chickens neutralizing antibodies egg SARSCoV2 VirusesMDPI ucdavis UTSEngage GeorgeMasonU
By Dr. Priyom Bose, Ph.D.Jul 12 2022Reviewed by Benedette Cuffari, M.Sc. The severe acute respiratory syndrome coronavirus 2 was first reported in late December 2019 in Wuhan, China, and is the etiologic agent of the ongoing coronavirus disease 2019 pandemic. To date, SARS-CoV-2 has infected over 557 million and claimed more than 6.35 million lives worldwide.
Study: Hyperimmunized Chickens Produce Neutralizing Antibodies against SARS-CoV-2. Image Credit: ustas7777777 / Shutterstock.com In coronaviruses, the RBD is the most antigenic site of the S1 domain and, as a result, is used as the target in current COVID-19 vaccines and most therapeutics. The S1 domain of the protein induces the production of neutralizing antibodies.
Studies have shown that an egg yolk typically yields 50-100 mg of IgY, which contain between 2-10% of specific antibodies. However, the amount of antigen-specific IgY produced by hyperimmunized hens depends on the age of the hen, dose, antigenicity, molecular weight of the antigen, and route of administration.
In this study, all vaccines had a volume of 0.5 µl and were administered through the intramuscular route in the pectoral muscle. Blood samples were collected 21 days following the second immunization and at the end of the experiment, which was six weeks after the second dose. The dose and type of antigen were the two most important factors that influenced the number of antibodies produced. Although all vaccines produced antibodies against SARS-CoV-2, vaccines that targeted the glycosylated S1 protein and a combination of glycosylated S1 and non-glycosylated RBD of S1 exhibited better neutralization capacity. Notably, antibodies generated from vaccine C could be administered as a fraction of the dose necessary for an effective seroconversion.
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