Just Stop Oil said it was in protest at the government's plans to licence new oil and gas projects.
Oxford University said the main entrance and exit to the reading room were closed because of a Just Stop Oil protest shortly after midday.
The group said student supporters painted the building in protest at the government's plans to licence new oil and gas projects.Thames Valley Police said it received reports of criminal damage on Radcliffe Square in Oxford at about 12:25 BST. The force said a 21-year-old man, from Oxford, and a 18-year-old man, from Norwich, had been detained and remained in police custody.
The building, known as the Rad Cam, was built in the 1730s and is used as a reading room by the Bodleian Library.
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