A crucial step in the degradation and utilization of lignin is the process of O-demethylation of lignin monomers, facilitated by O-demethylases. Current O-demethylases face challenges such as limited substrate specificity, unclear reaction mechanisms, and a lack of reductase chaperones.
Researchers reveal novel mechanism of enhanced P450 demethylase activity through engineered key gating residues retrieved 14 June 2024 from https://phys.org/news/2024-06-reveal-mechanism-p450-demethylase-key.html
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