Science, Space and Technology News 2024
This illustration represents a bacteria being illuminated with mid-infrared in the top left, while visible light from a microscope underneath is used to help capture the image. Credit: 2024 Ideguchi et al./ Nature Photonicshave developed an advanced mid-infrared microscope, enabling them to see the structures inside living bacteria at the nanometer scale. Mid-infrared microscopy is typically limited by its low resolution, especially when compared to other microscopy techniques.
The microscopic realm is where viruses, proteins, and molecules dwell. Thanks to modern microscopes, we can venture down to see the inner workings of our very own cells. But even these impressive tools have limitations. For example, super-resolution fluorescent microscopes require specimens to be labeled with fluorescence. This can sometimes be toxic to samples and extended light exposure while viewing can bleach samples, meaning they are no longer useful.
The team used a “synthetic aperture,” a technique combining several images taken from different illuminated angles to create a clearer overall picture. Typically, a sample is sandwiched between two lenses. The lenses, however, inadvertently absorb some of the mid-infrared light. They solved this issue by placing a sample, bacteria , on a silicon plate which reflected visible light and transmitted infrared light.
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Improved mid-infrared nanoscopy enables 30 times clearer view of the insides of bacteriaA team at the University of Tokyo have constructed an improved mid-infrared microscope, enabling them to see the structures inside living bacteria at the nanometer scale. Mid-infrared microscopy is typically limited by its low resolution, especially when compared to other microscopy techniques. Their work has been published in Nature Photonics.
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