In this article, InProcess-LSP compares spatially resolved DLS and conventional DLS.
Sponsored Content by InProcess-LSPReviewed by Louis CastelNov 6 2024 Author: Bas Evers, Biopharmaceutical Application Specialist, InProcess-LSPThe use of nanoparticles has become increasingly popular in multiple industries, such as the chemical, food and pharmaceutical industries. Applications range from quantum dots in tv-screens to lipid nanoparticles in the recently developed mRNA covid-19 vaccines.
SR-DLS offers a major benefit compared to DLS by being able to measure significantly more turbid samples without the need to dilute. Both DLS and SR-DLS require the scattered light to originate from single scattering effects in order to determine particle size. In contrast to conventional DLS, SR-DLS measures dynamic light scattering at multiple depths in the sample and can identify at what depth in the sample multiple scattering occurs and automatically adjust the pathlength accordingly.
The use of SR-DLS enables you to measure your suspension of interested directly in the vessel it is in, without direct contact with the suspension at all. For example, an end product contained in a syringe or vial can non-invasively be analysed. For stability testing for example, the same syringe or vial can therefore be analysed at different moments in time, which saves a significant number of stability samples and provides stability data of the same sample.
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