Study suggests potential therapeutic targets for COVID-19-related neurological pathologies Coronavirus Disease COVID Neurology CerebrospinalFluidProtein CSF molcellprot Tulane TulaneNPRC
Study: Cerebrospinal Fluid Protein Markers Indicate Neuro-Damage in SARS-CoV-2-Infected Nonhuman Primates. Image Credit: DOERS / Shutterstock
While more chronic neurological complications have been associated with severe COVID-19 cases, cognitive impairment has also been observed in mild cases. The use of mass spectrometry in investigating the proteins in CSF has proven to be an efficient method for understanding the mechanisms of neurodegenerative disorders. Cerebrospinal fluid is an ideal sample choice since obtaining it is comparatively less risky and invasive than biopsies of the brain.
Two African green monkeys and two rhesus macaques were used as age-matched controls, and a negative SARS-CoV-2 test for all the animals was ensured before the commencement of the study. Whole blood and aseptic CSF were obtained from all the animals after being infected with SARS-CoV-2. Additionally, sections of the cerebellum, brain stem, and basal ganglia were analyzed using immunohistochemistry, while additional sections of the CNS, such as the temporal, occipital, parietal, and frontal lobes and upper, lower, intermediate, and middle lobes of the lungs were used to quantify the CNS and pulmonary pathologies in non-human primates infected with SARS-CoV-2. Enzyme-linked immunosorbent assays were also conducted to analyze the plasma matrix metalloproteinase-2.
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