New approach for treating Alzheimer's shows success in mouse models
] by measuring the decrease in the exploratory activity of an animal in a test session carried out 24 h after the first exploration session . Animals were placed in a 40 cm × 50 cm × 60 cm open field box for a 5-min period. Twenty-four hours later, animals were re-exposed to the same environment for a 5-min period. The time in a mobile state and the distance traveled were measured in both sessions using EthoVision XT . A larger delta between days represented intact learning.
For IF staining, following incubation with the primary antibody, the fluorophore-conjugated secondary antibodies listed in Additional file: Table S2 were used. The cell nuclei were stained with DAPI . Sections were mounted with fluoroshield mounting medium , and viewed with an Olympus IX81 confocal microscope. Quantification of staining intensities was performed using Image J, with five randomly selected images examined in each section.
Protein levels were quantified based on the staining intensity in images selected over the whole area of the section using Image J software. Data were exported to Excel for statistical analysis. The background staining intensity for each image was subtracted from the total staining intensity.Behavioral data were analyzed using one-way ANOVA, followed by Tukey’s post-hoc analysis. An average value was determined for each group, and data are expressed as mean ± SD.
Previously, we have shown that VDAC1 overexpression leads to its oligomerization followed by apoptosis [
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