One child was pronounced dead at the scene on Saturday morning while a second child died overnight in hospital.
Five people were taken to hospital following the blaze at an address on Napier Road, East Ham , with those involved believed to be members of the same family.
The cause of the fire is not yet known. Police said there is currently no evidence to indicate suspicious circumstances.“This is a terribly sad incident and the thoughts of all of us at London Fire Brigade are with the family, friends and all those affected at this difficult time," London Fire Brigade Assistant Commissioner, Patrick Goulbourne, said.
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