Three-dimensional in vitro model to reproduce COVID-19-induced endothelial dysfunction biorxivpreprint UTAustin COVID19 SARSCoV2 EndothelialDysfunction
By Pooja Toshniwal PahariaOct 5 2022Reviewed by Aimee Molineux In a recent study posted to the bioRxiv* preprint server, researchers developed a three-dimensional in vitro tissue model to assess the effects of severe acute respiratory syndrome coronavirus 2 on the vascular endothelial cells.
About the study In the present study, researchers extended their previous analysis by creating a 3D model to study vascular network disruption in COVID-19. The cells were also treated with dexamethasone and visualized by confocal microscopy. The vessel length, vascular connectivity, and vessel lumen diameters were analyzed based on a previously developed computational pipeline. SARS-CoV-2 messenger ribonucleic acid from the hydrogels was subjected to qRT-PCR analysis to quantify mRNA expression based on the cycle threshold values.
Following CSP treatment, the vascular network showed reduced density and more cells with an altered rounded morphology. CSP treatment led to 22%, 33% and 29% reductions in vessel endpoints, vessel branch points, and vessel links, respectively. The corresponding reductions observed on using CSP in 100 µg/mL concentration were 17%, 24%, and 22%, respectively.
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