Transcreener cGAMP cGAS Assay directly measures cyclic GMP-AMP (cGAMP) produced by cyclic GMP-AMP synthetase (cGAS, C6orf150, 2'3'-cGAMP Synthetase).
The Transcreener cGAMP cGAS Assay relies on direct detection of cGAMP produced by cGAS to measure enzyme activity. The assay uses antibodies that selectively bind cGAMP in the presence of excess ATP and GTP. When the fluorescent tracer is displaced by cGAMP, a change in signal is achieved. The novel direct detection method simplifies the protocol and minimizes compound interference.cGAS titration at 1 hour and 24 hours demonstrates the assay's oustanding signal stability.
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