Research team shines light on how the brain stores positive and negativememories CommsBio NatureComms
, for 20 minutes and then cooled to room temperature. Two-photon imaging system was used to distinguish appetitive and aversive cells. Imaging system is equipped with a mode-locked Ti:Sapphire laser which was set to wavelengths between 920 nm to 950 nm in order to excite Alexa Fluor 488 and 568, tdTomato and EYFP fluorophores using a 20x, NA 1.0 objective lens .
Comparison between appetitive and aversive memory cells were done both within a single slice, within an animal as well as across animals to control for potential differences between animals or slices. Since the results in all three situations were similar, the pooled data for comparison of different groups d’Agostino-Pearson K2 test was used to determine normality of data.
United Kingdom Latest News, United Kingdom Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
3 Positives and 3 Negatives from Fulham 1 Newcastle 4The Editor asked me to give 3 positives and 3 negatives from Fulham 1 Newcastle 4, so here they are...
Read more »
Single-cell multi-omics profiling links dynamic DNA methylation to cell fate decisions during mouse early organogenesis - Genome BiologyBackground Perturbation of DNA methyltransferases (DNMTs) and of the active DNA demethylation pathway via ten-eleven translocation (TET) methylcytosine dioxygenases results in severe developmental defects and embryonic lethality. Dynamic control of DNA methylation is therefore vital for embryogenesis, yet the underlying mechanisms remain poorly understood. Results Here we report a single-cell transcriptomic atlas from Dnmt and Tet mutant mouse embryos during early organogenesis. We show that both the maintenance and de novo methyltransferase enzymes are dispensable for the formation of all major cell types at E8.5. However, DNA methyltransferases are required for silencing of prior or alternative cell fates such as pluripotency and extraembryonic programmes. Deletion of all three TET enzymes produces substantial lineage biases, in particular, a failure to generate primitive erythrocytes. Single-cell multi-omics profiling moreover reveals that this is linked to a failure to demethylate distal regulatory elements in Tet triple-knockout embryos. Conclusions This study provides a detailed analysis of the effects of perturbing DNA methylation on mouse organogenesis at a whole organism scale and affords new insights into the regulatory mechanisms of cell fate decisions.
Read more »
DNA methylation signatures of Alzheimer’s disease neuropathology in the cortex are primarily driven by variation in non-neuronal cell-types - Nature CommunicationsHere the authors identify differences in cortical DNA methylation associated with Alzheimer’s disease pathology, and profiling nuclei from specific cell-types, find that most of these differences reflect variation occurring in non-neuronal cells.
Read more »
'Skinometer' can detect skin cancer that's invisible to the human eyeExperts say some cancerous cells can be left behind during surgery - but it's hoped this technology will improve accuracy and slash operating times, alleviating pressure on the NHS.
Read more »
Gangland killer Paul Lyons 'loses parole' over iPhone at high security prisonHamilton Sheriff Court heard earlier this week the 41-year-old's cell was searched by jail guards who found the phone stashed inside a hidden compartment of a box.
Read more »
Communication key for Liz Truss as MPs gather for Tory conferenceAs the Tory party conference starts, MPs, the market and the public need reassurance, the BBC's Ione Wells says.
Read more »