Researchers identify how HIV/hepatitis drug harms the kidneys
MetaboAnalyst was used to perform gene-metabolite interaction network analysis on differentially expressed metabolites and genes—identified by metabolomics and transcriptomics. The network was refined by reducing the number of less important genes and metabolites by applying a degree filter of 5.Cells were lysed in the presence of a protease inhibitor cocktail and homogenized.
Serial 4 µm sections were air dried, deparaffinized in Histoclear, and washed in water. Sections were rehydrated in a graded ethanol series . Antigen retrieval was performed with 1 mEDTA pH 8.0 buffer for 40 min. Sections were incubated in 10% goat serum for 1 h and were then covered in a primary antibody cocktail for either complex I/IV or III/V , in a humidified chamber overnight. Following washes, sections were incubated in a secondary antibody cocktail for 2 h.
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