Isolated monoclonal antibodies targeting SARS-CoV-2 S1/S2 cleavage effective against Delta and Omicron Antibodies Coronavirus Disease COVID Delta Omicron SARSCoV2 SciReports UTokyo_News OsakaU_Research gunma_uni_ad
By Pooja Toshniwal PahariaNov 28 2022Reviewed by Benedette Cuffari, M.Sc. In a recent Scientific Reports journal paper, researchers isolated 7G7 and NIBIC-71 from peripheral blood mononuclear cells derived from coronavirus disease 2019 convalescent individuals. These monoclonal antibodies were found to neutralize the severe acute respiratory syndrome coronavirus 2 Delta and Omicron variants.
About the study In the present study, researchers isolated two mAbs, 7G7 and NIBIC-71, that neutralized SARS-CoV-2 VOCs through different neutralization mechanisms. Recombinant mAbs NIBIC-71, 7G7/λ, and 7G7/κ were prepared to characterize 7G7 and NIBIC‑71. In addition, s-ACE2 interactions were assessed using ELISA.
Pulmonary tissues of the infected hamsters were obtained and subjected to histological analysis. These tissues were also used to determine the pulmonary viral load through CPE and reverse transcription-polymerase chain reaction assays. The NIBIC-71 mAb was derived from immunoglobulin G1 /Igκ with Ig Kappa variable 1-9 and Ig heavy variable 3-53 genes. The 7G7 mAb was derived from IgG1/Igλ2 with Ig lambda variable 3-25 and IGHV3-33 genes.
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