A common metabolite may help treat autoimmunediseases HokkaidoUni NatureComms
. For each T cell differentiation, subset-specific antibodies and cytokines were further supplemented. For Th1 differentiation, cells were cultured with anti-IL-4 antibody and IL-12 . For Th2 differentiation, cells were cultured with anti-IFNγ antibody and IL-4 . For Th17 differentiation, cells were cultured with anti-IL-4 antibody , anti-IFNγ antibody , IL-6 , and TGF-β . For Treg differentiation, cells were cultured with anti-IL-4 antibody , anti- IFNγ antibody , IL-2 , and TGF-β .
After blocking, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-mouse NRF2 , anti-mouse HO-1 , and anti-β-actin ; β-actin was used as an endogenous control. Afterward, horseradish peroxidase-conjugated anti-rabbit and anti-mouse antibodies were used as secondary reagents. Signals were detected using enhanced chemiluminescence western blot detection reagents and the LAS-4000 imaging system .
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