Scientists discover that metabolic sensor may play role in Alzheimer'sdisease JCI_Insight
). Two-way repeated measures ANOVAs were used to analyze differences of between-subject factors , and post hoc analyses were performed for assessing specific group comparisons.Western blot analysis was used to measure levels of APP and the CTFs CTF-α and CTF-β produced from cleavage of APP by α-secretases or β- and γ-secretases, respectively.
Sample mixtures were then spun down at 12,000for 10 minutes at 4°C. The aqueous layer of RNA was then carefully removed, gently mixed with 70% EtOH/original volume of QIAzol , and run through an RNeasy spin column according to manufacturer’s protocol.A total of 1 μg of RNA was then converted to cDNA using High-Capacity cDNA Reverse Transcription kit according to manufacturer’s protocol.
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