Study reveals mechanism involved in neuropathicpain, could help develop specific treatment jclinicalinvest NatureNeuro
Mechanical nociceptive threshold was evaluated before and up to 24 hours after intrathecal injection of equimolar doses of kynurenine , 3-hydroxykynurenine , 3-hydroxyanthranilic acid , or vehicle in WT mice . This panel shows representative curves obtained in the dose-response analysis of mechanical allodynia caused by equimolar doses of Kyn, 3-Hk, 3-Haa in WT mice . Time course of 3-Hk levels in the ipsilateral dorsal horn of the spinal cord of mice after sham and SNI surgeries .
Western blotting analysis of KMO expression in the ipsilateral dorsal horn of the spinal cord after sham or SNI surgery . Representative image of KMO expression analyzed by immunofluorescence in the dorsal horn of the spinal cord after sham or SNI induction . Scale bar: 50 μm. Mechanical nociceptive threshold was determined before and 14 days after SNI. Mice were then treated intrathecally with Ro 61-8048 or vehicle and mechanical allodynia was measured up to 7 hours after treatments .
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