Rethinking the protein inhibitor approach to cancer therapy stonybrooku
TR for TX Red. Each image was captured under the same exposure time and exported under the same scale of Look Up Table.For each sample, newly thawed cells were cultured for one passage before the experiment. Next, around 50,000–80,000 cells harvested from 80% confluent T-25 flasks were plated into 24-well plates, with three technical replicates for each inducer concentration. Dox was added into each well to obtain concentrations ranging from 0.01 ng ml.
To determine the reproducibility of gene expression histograms, cells were freshly thawed and incubated for the first week before flow cytometry testing. Then, we performed the dose–response measurements for three technical replicates as described above repeatedly over 4 weeks for each sample at several selected dox concentrations and compared the fluorescence intensity means between measurements from three timepoints.
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