Researchers report a heterologous vaccination strategy against IBV in Chickens MDPIOpenAccess UWMadison vaccine vaccination virus immunity
By Pooja Toshniwal PahariaFeb 2 2023Reviewed by Danielle Ellis, B.Sc. In a recent study published in Vaccines, researchers reported on the immunogenicity of a heterologous vaccine regimen against IBV , comprising prime doses of QAC -encapsulated plasmid and a booster dose of MVA .
The team prepared CEF from embryos of SPF white leghorn embryonated chicken eggs to confirm IBV Arkansas DPI strain expression from the vaccine construct. The constructs were prepared using p-CAGN-loaded QAC nanoparticles, modified vaccinia Ankara expressing nucleocapsid, and tetramethylbenzidine substrates. The QAC-encapsulated plasmid DNA/modified vaccinia Ankara-nucleocapsid construct efficacy was assessed among four groups of chickens.
The severity scores for IBV infection signs were documented once daily for all chickens over eight days following the challenge. Tracheal swab specimens and lachrymal fluid specimens were assessed for IBV ribonucleic acid by infectious bronchitis virus nucleocapsid gene-targeted quantitative reverse transcription-polymerase chain reaction analysis.
IBV-targeted immunoglobulin -A and -Y were significantly greater in the modified live virus-immunized chickens compared to the non-immunized PBS control chicken group. However, the Ig titers were not significantly greater among chickens vaccinated with either vaccine regimen compared to non-vaccinated chickens.
The heterologous doses of QAC-encapsulated plasmid DNA/modified vaccinia Ankara-nucleocapsid vaccines provided significantly greater protection against IBV than the homologous 2.0X modified vaccinia Ankara-nucleocapsid doses. The immune protection may be due to the robust cell-mediated memory response induction regulated by QAC-encapsulated plasmid DNA/modified vaccinia Ankara-nucleocapsid in pulmonary tissues.
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