How a SARS-CoV-2 virus protein damages the heart ummedschool nature CommsBio
expression. Adult male flies were maintained in vials in groups of 20 or fewer. The number of living flies in each group was recorded every second day. The assay was carried out until no survivors were left for any of the fly lines. Per genotype 100 flies were assayed.Adult flies were dissected and fixed for 10 min in 4% paraformaldehyde in phosphate-buffered saline . Flies were incubated in block solution containing 0.
was used to process the images. The diastolic dimension and systolic diameter were processed, measured, and determined based on three consecutive heartbeats. The heart rate was determined by counting the total number of beats that occurred during a 15-second recording.For each sample, hearts from fifty-one—week-old adult flies were manually dissected and collected directly into ice-cold TRIzol LS reagent .
Illumina-based RNAseq library preparation and subsequent paired-end sequencing were performed by Novogene . Novogene also carried out mapping of the short-reads, their quantification, and initial differential analyses. In brief, reads that contained Illumina-adaptor sequences or those considered to be of poor quality were removed before mapping. The short-reads, generated from the RNAseq, were mapped to the Berkeley Drosophila Genome Project reference genome release 6 using HISAT2 version 2.0.
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