The roles of APOBEC-mediated RNA editing in SARS-CoV-2 mutations, replication and fitness - Scientific Reports

COVID-19 mutations accelerated by virus-fighting enzyme in humancells, according to new research USC SciReports

We used lentiviral transfection to construct stable Caco-2 cell lines expressing A3A, A3G, and A1 + A1CF to study the effect of APOBEC on SARS-CoV-2 replication because Caco-2 expresses the virus receptor ACE2

The ∆A3A Caco-2 cell line was created by puromycin selection after targeting N-terminus of genomic A3A exon-2 region with CRISPR-Cas9 methods and inserting the SV40-promoter-Puromycin resistant gene through homology directed repair system . A randomized guide RNA was used to generate a Caco-2 cell line as a negative control.

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