Advanced nanoparticle detection methods like electron microscopy and DLS are key for characterizing size, shape, and composition in nanotechnology.
By Rebecca Ingle, Ph.DMay 2 2024Reviewed by Lexie Corner Nanoparticles are objects with a diameter ranging from 1 to 100 nm.1 They have a myriad of applications in medical sciences, photonics, chemical synthesis, and catalysis.2-5 A crucial element in both the synthesis and functionality of nanoparticles involves the attachment of ligands.6 The appropriate choice of ligands can enhance the solubility of nanoparticles or affect their aggregation and self-assembly behavior.
However, optical detection techniques like absorption, fluorescence, and surface plasmon resonant methods can still provide indirect size information and be valuable tools for profiling the optical properties of nanoparticles. Many nanoparticles are used to generate surface plasmon resonance applications, such as cell detection or biomolecular sensing.11 However, SPR can also be used to detect nanoparticles themselves.
Mass Spectrometry Mass spectrometry is a useful tool in nanoparticle detection, aiding in the identification of a sample’s elemental composition. It is also used to understand the repeating units that comprise a nanoparticle and their relative stability.13
Electron Microscopy Optical Detection Mass Spectrometry Dynamic Light Scattering Field-Flow Fractionation
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