Monoclonal antibody-based blocking ELISA for SARS-CoV-2 detection in animals Antibody ELISA Monoclonal Antibody SARSCoV2 Coronavirus Disease COVID UofIllinois Cornell biorxivpreprint
By Bhavana KunkalikarMar 16 2023Reviewed by Danielle Ellis, B.Sc. *Important notice: bioRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information.
About the study In the present study, researchers described the generation of a blocking ELISA based on monoclonal antibodies to identify SARS-CoV-2 exposure among animals. SARS-CoV-2 was experimentally introduced into a group of 24 cats. Cat serum samples were collected 14 days after infection and were combined to create a group of positive control serums. Also, large volumes of negative cat sera were combined to create a single batch of negative control serum. To investigate the diagnostic sensitivity as well as specificity associated with mAb-based bELISA, the team evaluated a panel of blood samples from cats, ferrets, minks, and deer with known antibody status.
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